Evaluation of intracellular telomerase activity through cascade DNA logic gates† †Electronic supplementary information (ESI) available: Sequences used in this study, fluorescence spectroscopy of logic gate activation using synthetic TS oligonucleotide with different numbers of elongation repeats, flow cytometry data, confocal images of counter staining, time course, control samples, and L-02 and Hep G-2 cells. See DOI: 10.1039/c6sc01953f Click here for additional data file.
نویسندگان
چکیده
State Key Laboratory of Analytical Chem Innovation Center of Chemistry for Life Scie Engineering, Nanjing University, Nanjing 2 cn; [email protected] School of Chemistry and Life Science, Na 210093, China School of Medical Imaging, Xuzhou Medica School of Pharmacy, Ningxia Medical Univ College of Chemistry and Chemical Enginee Marine Biomass Fiber Materials and Texti China † Electronic supplementary information study, uorescence spectroscopy of log oligonucleotide with different numbers data, confocal images of counter stainin L-02 and Hep G-2 cells. See DOI: 10.1039/ Cite this: Chem. Sci., 2017, 8, 174
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Rapid engineering of versatile molecular logic gates using heterologous genetic transcriptional modules† †Electronic supplementary information (ESI) available: Supplementary methods, gene regulatory sequences used, the characterised dose responses of the single-input Buffer gate and the three promoter inputs under various cognate chemical induction levels. See DOI: 10.1039/c4cc05264a Click here for additional data file.
We designed and constructed versatile modular genetic logic gates in bacterial cells. These function as digital logic 1-input Buffer gate, 2-input and 3-input AND gates with one inverted input and integrate multiple chemical input signals in customised logic manners. Such rapidly engineered devices serve to achieve increased sensing signal selectivity.
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عنوان ژورنال:
دوره 8 شماره
صفحات -
تاریخ انتشار 2017